Chronichydrogen-rich saline treatment attenuates vascular dysfunction in spontaneoushypertensive rats
HaoZhenga, 1, Yong-Sheng Yub, 1, ,
aDepartment of Pharmaceutical Science and Technology, College of Chemistry andBiology, Donghua University, Shanghai, China
The 8-week-old spontaneously hypertensive rats (SHR) and age-matched Wist ar-Kyotorats (WKY) were randomized into HRS-treated (6 ml/k). In hypertensive patients,increased oxidative stress is thought to be one important cause of vasculardysfunction. Recently, it has been suggested that hydrogen exerts a therapeuticantioxidant activity by selectively reducing hydroxyl radical andperoxynitrite, the most cytotoxic chemicals of reactive oxygen species (ROS).Herein, we investigated the protective effect of chronic treatment withhydrogen-rich saline (HRS) against vascular dysfunction in SHR and theunderlying mechanism.
Treatmentwith HRS ameliorated vascular dysfunction including aortic hypertrophy andendothelial function in SHR.
Treatmentwith HRS had no significant effect on blood pressure, but it signi ntlyimproved baroreflex function in SHR. Treatment with HRS abated oxidativestress, restored antioxidant enzymes including superoxide dismutase,glutathione peroxidase, and catalase, and suppressed NADPH oxidase.Furthermore, treatment with HRS depressed pro-inflammatory cytokines expressionincluding IL-6 and IL-1β and suppressed NF-κB activation, restoredmitochondrial function including ATP formation and membrane integrity. Inaddition, although treatment with HRS had no significant effect on nitric oxideamount in circulating or aorta, it suppressed endothelial nitric oxide synthaseexpression and upregulated dimethylarginine dimethylaminohydrolase 2 expressionin SHR. In conclusion, treatment with HRS alleviates vascular dysfunctionthrough abating oxidative stress, restoring baroreflex function, suppressinginflammation, preserving mitochondrial function, and enhancing nitric oxidebioavailability.